Products & Services

Introduction

Virovek provides a range of high-quality viral vector manufacturing and purification services, including AAV, lentivirus, baculovirus, and adenovirus systems, as well as plasmid and sterile bacmid production, for gene therapy research. Each service offers customization, scalability, and high purity, backed by extensive quality control measures like PCR, electrophoresis, and DNA sequencing to ensure reliable performance. These solutions cater to both off-the-shelf and custom needs, accelerating clinical research and biomanufacturing with quick turnaround times and high yields.

AAV Vector Services

We offer both off-the-shelf AAV vectors of various serotypes that encode commonly used reporter genes for quick turn-around, as well as custom-made vectors tailored to your specific research needs or clinical requirements. Our services ensure:

  • High Purity: Advanced purification techniques guarantee high-quality vectors.
    • Double cesium chloride (CsCl) ultracentrifugation
    • Affinity-mediated column chromatography
  • Customization: Personalized vector design for specific gene expression and enhanced viral tropism can be combined with our extensive selection of promoters, poly-adenylation (polyA) signals, and serotypes.
  • Scalability: Productions range from 1E+13 vector genomes (vg) to 1E+17 vg per batch
  • Quality Control: Extensive assay panels allow product validation that ensures consistent performance:
    • Titer determination using:
      • Quantitative PCR (qPCR)
      • Digital PCR (dPCR)
      • Nanodrop
      • Capsid ELISA
    • Purity evaluation using SDS-PAGE
    • Genome size confirmation using agarose gel electrophoresis
    • DNA Sequencing of AAV vector
    • Full/empty capsid evaluation using capillary electrophoresis
    • Endotoxin measurement
    • Aggregation assessment using dynamic light scattering
    • Osmolality determination
    • Transduction efficiency evaluation using flow cytometry
    • Infectivity assessment via tissue culture infectious dose 50 (TCID50) assay
    • Replication competent (rc) AAV detection
    • Certificate of Analysis (CoA)

Ideal for accelerating your gene therapy research or product development.

AAV-Vector-Tech
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Lentivirus Vector Services

We supply both off-the-shelf lentiviral vectors containing various promoters and commonly used genes for immediate application, as well as custom-made vectors designed to meet your unique research goals or clinical needs. Our services include:

  • High Transduction Efficiency: Our purified or non-purified vectors are optimized for superior gene delivery across numerous cell types.
  • Customization: Tailored vector design ensures specific gene expression, promoter selection, or genetic modifications and is verified by DNA sequencing.
  • Improved Yields: Offer high viral titers (>1E+10 TU/ 100 mL suspension culture) through optimized reagents and culture conditions.
  • Quick Turnaround: 2-3 weeks (from order placement to delivery) for custom vectors.
  • Quality Control: Extensive and precise testing ensures high viral titer, purity, and functionality:
    • Titer determination using:
      • Reverse transcriptase quantitative PCR (qPCR)
      • Reverse transcriptase digital PCR (dPCR)
      • p24 ELISA
    • Purity evaluation using SDS-PAGE
    • Genome size confirmation using RNA gel electrophoresis
    • Transduction efficiency evaluation using flow cytometry
    • Certificate of Analysis (CoA)

Perfect for advancing your clinical research or developing novel gene therapies.

Baculovirus Production Services:

We offer advanced baculovirus manufacturing services using both the Bac-to-Bac™ and Homologous Recombination systems for:

  • High Fidelity: Exceptionally accurate insertion of a gene of interest (GOI) into the baculovirus genome minimizes mutations and is verified by DNA sequencing analysis.
  • High Yield: Optimized for producing high titers of recombinant baculovirus in insect cells.
  • Versatility: Suitable for diverse applications, ranging from vaccines to therapeutic proteins. We provide non-concentrated or concentrated products, and plaque-purified or non-plaque-purified seed stocks.
  • Scalability: Efficient scaling from research to industrial production, without compromising the critical quality attributes of manufactured products.
  • Quality Control: Rigorous testing ensures high baculovirus titer, purity, and functionality:
    • Titer determination using quantitative PCR (qPCR)
    • GOI verification by PCR amplification and DNA analysis by agarose gel electrophoresis
    • DNA sequencing to verify GOI
    • Infectivity assessment using tissue culture infectious dose 50 (TCID50) assay
    • Certificate of Analysis (CoA)

Leverage our services for rapid, reliable baculovirus vector production tailored to your research or biomanufacturing needs.

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AdONE-Adenovirus-Generation

AdONE Adenovirus Generation System

Utilizing our proprietary technology, we produce adenoviral products that feature:

  • One-Day Process for Transfection: Dramatically reduces the time from gene insertion into the adenoviral genome to cell transfection.
  • High Efficiency: Ensures rapid, high-yield production of adenoviral vectors.
  • Customization: Designed to incorporate your gene of interest (GOI) directly into the adenoviral genome and transfect cells in a single day to generate adenovirus.
  • Flexibility: Tailored to your needs by providing both crude cell lysates and/or purified adenovirus. We purify adenoviral vectors through double cesium chloride (CsCl) ultracentrifugation, followed by buffer exchange using standard or custom-made buffers.
  • Quality Control: Delivers adenovirus of high purity and titer, that is validated for research or clinical applications:
    • Titer determination using:
      • Quantitative PCR (qPCR)
      • Nanodrop
    • Purity evaluation using SDS-PAGE
    • Genome size confirmation using agarose gel electrophoresis
    • DNA extraction for analysis by restriction enzyme digestion
    • Infectivity assessment using:
      • Tissue culture infectious dose 50 (TCID50) assay
      • Plaque assay
    • Certificate of Analysis (CoA)

Our system streamlines the adenovirus manufacturing process, offering speed, precision, and reliability.

Plasmid Purification Services

We offer scalable plasmid purification from 100 μg to 100 mg under sterile or non-sterile conditions with:

  • High Purity: Over 90% of the DNA is supercoiled.
  • Endotoxin-Free: Ensures minimal endotoxin levels to enable plasmid use in endotoxin-sensitive applications like transfection or gene therapy.
  • Quality Control: Delivers plasmid DNA of high purity that is validated for research or therapeutic applications:
    • DNA concentration using Nanodrop
    • DNA purity and protein impurities using the OD260/OD280 ratio
    • Whole plasmid DNA sequencing verification
    • Plasmid analysis using restriction enzyme digestion and agarose gel electrophoresis
    • Endotoxin removal
    • Certificate of Analysis (CoA)

Our service delivers high-quality, endotoxin-free plasmid DNA tailored to meet your research or production needs.

Plasmid-Purification
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Sterile Bacmid Purification Services

We offer scalable purification of bacmid DNA from 10 μg to 10 mg with:

  • High Purity: Ensures that Bacmid DNA is suitable for efficient transfection.
  • Sterility: Sterility is maintained throughout the purification process, allowing immediate use of the Bacmid for the generation of recombinant baculovirus.
  • Quality Control: Delivers sterile Bacmid DNA of high purity that is validated for immediate transfection to generate recombinant baculovirus:
    • DNA purification using large construct columns
    • DNA concentration using Nanodrop
    • DNA purity and protein impurities using the OD260/OD280 ratio
    • Quantitative PCR (qPCR) amplification of gene of interest (GOI) and DNA analysis by agarose gel electrophoresis
    • DNA sequencing to verify GOI coding sequence
    • Certificate of Analysis (CoA)

Our service provides high-quality, sterile Bacmid DNA optimized for baculovirus production for your research or manufacturing processes.

The World’s Most Efficient Large-Scale AAV Manufacturing System

Virovek’s patented BAC-TO-AAV technology has the capability of generating up to 1E+17vg of adeno-associated virus in a single run. Learn more about our technology platform and explore a wide range of applications.

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Virovek-Lab-Team