Publications

Highly efficient neuronal gene knockout in vivo by CRISPR-Cas9 via neonatal intracerebroventricular injection of AAV in mice

Highly efficient neuronal gene knockout in vivo by CRISPR-Cas9 via neonatal intracerebroventricular injection of AAV in mice Sam Hana, Michael Peterson, Helen McLaughlin, Eric Marshall, Attila J. Fabian, Olivia McKissick, Kathryn Koszka, Galina Marsh, Michael Craft, Shanqin Xu, Alexander Sorets, Tess Torregrosa, Chao Sun, Chris E. Henderson, Shih-Ching Lo Gene Ther (2021)....

04 September, 2021

TPP Combined with DGUC as an Economic and Universal Process for Large-Scale Purification of AAV Vectors

Adeno-associated virus (AAV) vectors have been commonly purified through density gradient ultracentrifugation (DGUC) or column chromatography methods. Although the DGUC method can efficiently separate the empty from the full virus particles, its application in large-scale AAV purification is hindered due to its limitation in volume of each centrifuge tube. ...

02 August, 2020

Exploiting the intron-splicing mechanism of insect cells to produce viral vectors harboring toxic genes for suicide gene therapy

Exploiting the intron-splicing mechanism of insect cells to produce viral vectors harboring toxic genes for suicide gene therapy Chen H. Mol Ther Nucleic Acids. 2012 Nov 27;1:e57. ...

02 August, 2020

Intron splicing-mediated expression of AAV Rep and Cap genes and production of AAV vectors in insect cells

Intron splicing-mediated expression of AAV Rep and Cap genes and production of AAV vectors in insect cells Chen H. Mol Ther. 2008 May;16(5):924-30. Epub 2008 Mar 18. ...

02 August, 2020

Kinetics and MR-Based Monitoring of AAV9 Vector Delivery into Cerebrospinal Fluid of Nonhuman Primates

Here we evaluated the utility of MRI to monitor intrathecal infusions in nonhuman primates. Adeno-associated virus (AAV) spiked with gadoteridol, a gadolinium-based MRI contrast agent, enabled real-time visualization of infusions delivered either via cerebromedullary cistern, lumbar, cerebromedullary and lumbar, or intracerebroventricular infusion....

02 August, 2020

To transduce a zebra finch: interrogating behavioral mechanisms in a model system for speech

To transduce a zebra finch: interrogating behavioral mechanisms in a model system for speech Jonathan B. Heston, Stephanie A. White J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2017; 203(9): 691–706. Published online 2017 Mar 7. doi: 10.1007/s00359-017-1153-0 PMCID: PMC5589492 ...

02 August, 2020

TRPC3 channels critically regulate hippocampal excitability and contextual fear memory

TRPC3 channels critically regulate hippocampal excitability and contextual fear memory Sarah M. Neuner, Lynda A. Wilmott, Kevin A. Hope, Brian Hoffmann, Jayhong A. Chong, Joel Abramowitz, Lutz Birnbaumer, Kristen O’Connell, Andrew K. Tryba, Andrew S. Greene, C. Savio Chan, Catherine C. Kaczorowski Behav Brain Res. Author manuscript; available in PMC 2016 Mar...

02 August, 2020

Mutant huntingtin enhances activation of dendritic Kv4 K+ channels in striatal spiny projection neurons

Mutant huntingtin enhances activation of dendritic Kv4 K+ channels in striatal spiny projection neurons Luis Carrillo-Reid, Michelle Day, Zhong Xie, Alexandria E Melendez, Jyothisri Kondapalli, Joshua L Plotkin, David L Wokosin, Yu Chen, Geraldine J Kress, Michael Kaplitt, Ema Ilijic, Jaime N Guzman, C Savio Chan, D James Surmeier eLife. 2019; 8:...

02 August, 2020

Heparan Sulfate Organizes Neuronal Synapses through Neurexin Partnerships

Heparan Sulfate Organizes Neuronal Synapses through Neurexin Partnerships Peng Zhang, Hong Lu, Rui T. Peixoto, Mary K. Pines, Yuan Ge, Shinichiro Oku, Tabrez J. Siddiqui, Yicheng Xie, Wenlan Wu, Stephanie Archer-Hartmann, Keitaro Yoshida, Kenji F. Tanaka, A. Radu Aricescu, Parastoo Azadi, Michael D. Gordon, Bernardo L. Sabatini, Rachel O.L. Wong, Ann...

02 August, 2020

A role for cerebellum in the hereditary dystonia DYT1

A role for cerebellum in the hereditary dystonia DYT1 Rachel Fremont, Ambika Tewari, Chantal Angueyra, Kamran Khodakhah eLife. 2017; 6: e22775. Published online 2017 Feb 15. doi: 10.7554/eLife.22775 PMCID: PMC5340526 ...

02 August, 2020